phospho-jak2 (tyr1007/1008) (c80c3) rabbit mab antibody  (Cell Signaling Technology Inc)

Journal: Journal of Inflammation Research

Article Title: Wenqing Yin Attenuates the Allergic Contact Dermatitis Response by Inhibiting the JAK2/STAT1 and MAPK Signaling Pathways

doi: 10.2147/JIR.S512868

Figure Lengend Snippet: Impact of WQY on the JAK/STAT and MAPK signaling pathways in DFNB-induced ACD mice. At 24 h after the last challenge, the ear tissues of ACD mice were removed. The extracted proteins were quantified and separated by 10% SDS-PAGE, and the p -JAK2, JAK2, p -STAT1, STAT1, p -p38, and p38, p -JNK, JNK, p -ERK, and ERK expressions ( A ) were measured by Western blotting. Next, the analysis focused on the ratios of p-JAK2 to JAK2 and p-STAT to STAT1 ( B ), as well as the ratios of p-ERK to ERK, p-JNK to JNK, and p-p38 to p38 ( C ). Data are expressed as mean ± SD; n = 3; ## P < 0.01, # P < 0.05 versus the control group; * P < 0.05, ** P < 0.01 versus the model group.

Article Snippet: After being separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, the measured proteins were moved to polyvinylidene fluoride membranes and incubated with primary antibodies against p -JAK2 (CST, 3771S), JAK2 (CST, 3230S), p -STAT1 (CST, 7649S), STAT1 (CST, 14994S), p -p38 (CST, 4631S), p38 (CST, 8690S), p -JNK (CST, 4668S), JNK (CST, 9252S), p -ERK (CST, 4370S), and ERK (CST, 4695S)for an additional night at 4 °C, followed by a 2-hour incubation (37 °C) with the appropriate peroxidase-conjugated anti-rabbit IgG antibodies.

Techniques: Protein-Protein interactions, SDS Page, Western Blot, Control